CFX Connector
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CFX Connector
The 96-well block of the CFX Connect System offers excellent thermal
performance and uniformity across the entire block. Precision of the
temperature steps is critical for the rate and efficiency of PCR. To
obtain reliable, consistent results, all sample wells must maintain proper
temperature throughout each incubation step. The CFX Connect
System achieves precision by using six independently controlled thermal
electric modules, the heating and cooling elements of the thermal
cycler, to maintain tight temperature uniformity at all points during a
run — even while ramping. A high average ramp rate allows the system
to rapidly reach its target temperature, thus shortening run time with an
unsurpassed 10 sec settling time.
Efficient Optimization
Determining the optimal temperature for primer annealing is crucial for
efficient and specific target amplification. The thermal gradient feature of
the CFX Connect System quickly assists with optimizing your assay in a
single experiment, minimizing the use of precious samples and reagents
and saving valuable research time. At any step in a protocol, you can
program a temperature gradient of up to 24°C across the reaction block
with exceptional temperature uniformity and reproducibility within each
gradient zone.
The solid-state optical technology of the CFX Connect System enables
precise excitation and detection of fluorophores. Scanning just
above the sample plate, the optics shuttle, containing light-emitting
diodes (LEDs) and photodiodes, individually illuminates and detects
fluorescence from each well with high sensitivity and no cross talk.
The optical system automatically collects data from all wells during
data acquisition, so you can enter or edit well information on your
own schedule.