Chromatography
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- Fast protein liquid chromatography
Fast protein liquid chromatography
System Specifications
- Control system – ChromLab Software
(compatible across all NGC Systems) - Dimensions (W x D x H) – 49 x 61 x 56 cm (NGC Quest Plus and
NGC Scout Plus Systems)
49 x 61 x 74 cm (NGC Discover System) - Weight – 41–46 kg (NGC Quest Plus and
(excluding computer) NGC Scout Plus Systems)
64 kg (NGC Discover System) - Power supply – 100–240 V, 50–60 Hz
- Power consumption – 750 W maximum
System Pump
- Pump type – Reciprocating piston
- Flow rate setting – 10 ml/min pumps: 0.001–10 ml/min
(normal range)
100 ml/min pumps: 0.01–100 ml/min
(normal range) - Flow rate accuracy – ±2% (conditions: F10 pump — 0.1–10 ml/min,
F100 pump — 1.0–100 ml/min;
pressure: <600 psi [4.1 MPa, 41 bar];
viscosity: 0.5–3.7 cP) - Pressure range – 10 ml/min pumps: 0–25.2 MPa (3,650 psi)
100 ml/min pumps: 0–10 MPa (1,450 psi) - Viscosity range – 0.5–10.8 cP (for 10 ml/min and
100 ml/min pumps)
Sample Pump
- Pump type – Piston pump, metering type
- Flow rate setting – 0.01–100 ml/min
- Flow rate accuracy – ±2%
- Pressure range – 0–10 MPa (1,450 psi)
- Viscosity range – 0.5–10.8 cP
Mixer
- Mixing principle – Chamber with magnetic stirrer
- Mixer volume – 263 μl (included), 750 μl (included), 2 ml,
5 ml (F10)
750 μl (included), 2 ml (included), 5 ml,
12 ml (F100) - Gradient composition – ±0.5% (conditions: 3–97%B,
- accuracy – 0.25–10 ml/min F10 pumps)
±0.8% (conditions: 5–95%B,
1–100 ml/min F100 pumps)
Valves
- Type – Rotary valves and rocker solenoid
- Number of valves – 1 inject valve, up to 2 x 8-port sample
inlet valves, and 2 x 8-port buffer inlet,
2 x 12-port outlet, and 3 x 5-port column
switching valves - Functions – Loop selection
(PEEK Loop and DynaLoop offerings)
Pressure Sensors
- Placement of sensors Standard: after system pump
Options: precolumn, postcolumn,
sample pump - Range 0–3,650 psi
- Accuracy ±2 psi or 2%, whichever is greater
Inlet Valves
- Inlet A – 8 inlets
- Inlet B – 8 inlets
- Sample inlet – 8 or 15 inlets
Multi-Wavelength Detector II Module
- Wavelength – 190–800 nm
Up to 4 simultaneously - Absorbance range – 0–3.0 AU
- Linearity – 0–2.5 AU within ±5%
- Operating pressure – 700 psi (4.8 MPa)
- Flow cells – Preparative: 2 mm (volume: 140 μl)
Analytical: 5 mm (volume: 51 μl)
Analytical: 10 mm (volume: 24 μl) - Conductivity reading range – 0.01–999.9 mS/cm
- Accuracy – ±2%
- Operating pressure – 0–700 psi (0–4.8 MPa)
- Conductivity cell volume – Included in flow cell volume
- Temperature – 4–60°C monitor range
- Temperature – ±2% monitor accuracy
pH Monitor
- pH reading range – 0–14
- Accuracy – ±0.1 pH unit within pH 2–12
- Operating pressure – 0–70 psi with pH probe inline
and 0–500 psi in bypass mode - Flow cell volume – 100 μl (210 μl including internal flow paths)
Pressure Sensors
- Placement of sensors Standard: after system pump
Options: precolumn, postcolumn,
sample pump - Range 0–3,650 psi
- Accuracy ±2 psi or 2%, whichever is greater
NGC Fraction Collector
- Collection modes
Collect All, Threshold, and Time/Volume windows
Flow rate 0.01–200 ml/min - Collection rack options
(each NGC Fraction Collector can accommodate 4 racks)
96 x 13 mm tubes, 75 x 16 mm tubes,
75 x 18 mm tubes, 27 x 50 ml tubes,
2 x deep well microplates (24-/48-/96-well),
96 x 1.5–2 ml capless tubes, 16 x 250 ml
bottles, and 40 x unlimited volume
prep-rack adaptors - Operating temperature – 4–40°C
- Dimensions – (W x D x H) 42 x 60 x 54.5 cm
BioFrac Fraction Collector
- Collection modes
Time 0.02–99,999 min
Volume 0.02–99,999 ml
Flow rate 0.01–100 ml/min - Collection rack options 180 x 12–13 mm tubes, 120 x 15–16 mm
tubes, 80 x 18–20 mm tubes, 168 x 1.5 ml
microtubes, 24 x 30 mm tubes, 4 x 96-,
48-, 24-, or 12-position microplates, 4 x
250 ml bottles, and 20 x unlimited volume
prep-rack adaptors - Operating temperature 4–40°C
- Dimensions (W x D x H) 44.5 x 35.6 x 38.7 cm
Column Switching Valve
- Five-column valve – Can connect up to 5 columns with
forward and reverse flow, column bypass
capability, and column pressure readings
Buffer Blending Valve
- Blending valve – Standard in the NGC Scout Plus and
NGC Discover Systems
Automated inline buffer preparation from
concentrated buffer and salt stocks
Double the fluid output to 20 ml/min
or 200 ml/min
Air Sensor Module
- Number of sensors – Up to 8 total air sensors (1 for end of
sample detection, remaining are buffer) - Placement of End of buffer, end of sample
built-in sensors – - Sensing principle – Acoustic