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PCR

Real Time Thermal Cycler

The CFX Opus System is the next evolution in qPCR from Bio-Rad. With improved
thermal performance and our proprietary, accurate optical shuttle system, your data
will be more consistent than ever. A sleek, modern design includes a refreshed and
easy-to-use interface with new connectivity options and features.


In addition to ethernet compatibility, the CFX Opus System can connect directly to
Wi-Fi for completely wireless operation and data retrieval. The CFX Opus System is
capable of stand-alone operation and direct data transfer to a USB device, or it can
be connected to a computer.

With internet connectivity, the CFX Opus System can be programmed to send your
data and notifications directly to your email or local networked drive, enabling easy
access by collaborators or from other network-connected locations.
CFX Opus is available in three different block configurations: CFX Opus 96 for most
general qPCR applications, CFX Opus 384 for larger throughput needs, and CFX
Opus Deepwell with larger volumes up to 125 µl.

Model CFX Opus 384 System

Specifications:

  • Quick system set up — easy installation and factory-calibrated optics
  • Our best thermal uniformity and accuracy to date

MODEL CFX OPUS 96 WELL

Specifications:

  • Maximum ramp rate, °C/sec  CFX Opus 96 System: 5
  • Average ramp rate, °C/sec   CFX Opus 96 System: 3.3
  • Heating and cooling method   Peltier
  • Lid, °C 30–110

Temperature

  • Range, °C 4–100
  • Accuracy, °C ±0.2 of programmed target at 90°C
  • Uniformity, °C CFX Opus 96 and 384 Systems: ±0.3 well-to-well after 10 sec of arrival at Target

Gradient

  • Operational range, °C 30–100
  • Programmable span, °C 1–24

Optical Detection:

  • Excitation CFX Opus 96 System: 6 filtered LEDs
  • Detection CFX Opus 96 System: 6 filtered photodiodes
  • Range of excitation wavelengths, nm CFX Opus 96 System: 450–684
  • Range of emission wavelengths, nm CFX Opus 96 System: 510–730

Sensitivity

  • DNA-Detects 1 copy of target sequence in human genomic
  • Dynamic range10 orders of magnitude

Software

  • Sample capacity, wells 96-well and 384-well versions
  • Sample size, µl CFX Opus 96 System: 1–50 (10–50 recommended)
  • Communication interface USB 2.0
  • Electrical approvals IEC, CE
  • Dimensions (W x D x H), cm/in 33x56x36 / 13x22x14
  • Weight, kg/lb 22/48
  • Touch Screen Adjustable from 15°–22°
  • Embedded Operating System Windows 10 IoT

With internet connectivity, the CFX Opus System can be programmed to send your
data and notifications directly to your email or local networked drive, enabling easy
access by collaborators or from other network-connected locations.
CFX Opus is available in three different block configurations: CFX Opus 96 for most
general qPCR applications, CFX Opus 384 for larger throughput needs, and CFX
Opus Deepwell with larger volumes up to 125 µl.

Key Features and Benefits

  • Quick system set up — easy installation and factory-calibrated optics
  • Our best thermal uniformity and accuracy to date
  • Offers Wi-Fi connectivity and BR.io cloud platform analysis tools
  • Sensitive modern touch screen — responsive even with double gloves
  • Updated interface is easier to use and more intuitive than ever
  • Uniform optical system free of regular calibration requirements
  • Audit and e-signature compatibility with CFX Maestro Security Edition Software
  • Available in 96-well (5-color detection) and 384-well (4-color detection) formats

Specification

  • Maximum ramp rate, °C/sec  CFX Opus 96 System: 5
  • Average ramp rate, °C/sec   CFX Opus 96 System: 3.3
  • Heating and cooling method   Peltier
  • Lid, °C 30–110